genetic variations of avian pasteurella multocida as demonstrated by 16s-23s rrna gene sequences comparison
Authors
abstract
pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. a polymerase chain reaction (pcr) assay was developed using primers derived from conserved part of 16s-23s rrna gene. the pcr amplified a fragment size of 0.7 kb using dna from nine avian p. multocida isolates. sequence alignment of the 16s-23s rrna genes (its) revealed a considerable heterogenicity among the isolates. the percentage of similarity varied from 83.3 to 100% among the isolates. an interesting finding from this study was the presence of an inserted sequence (seven nucleotides) in the 16s-23s rrna region in 55% of the isolates. according to phylogenic analysis based on its sequence alignment, the p. multocida isolates classified into 2 distinct clusters. the virulence of isolates in cluster ii were higher than those in cluster i. ribotyping of p. multocida by using 16s-23s rrna gene pcr sequencing could be used as a marker in epidemiologic studies.
similar resources
Genetic variations of avian Pasteurella multocida as demonstrated by 16S-23S rRNA gene sequences comparison
Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates...
full textDiversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing
In this study, to increase information about the relationship between caprine and ovine isolates of Pasteurella multocida, 16S rRNA gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. Also, capsular type and toxAgene presentation was studied in this paper. All isolates, except one, belong to capsular type A, and toxA+ strain distributed among strains were isolated from both...
full textdiversity of caprine and ovine pasteurella multocida isolates based on 16s rrna gene sequencing
in this study, to increase information about the relationship between caprine and ovine isolates of pasteurella multocida, 16s rrna gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. also, capsular type and toxagene presentation was studied in this paper. all isolates, except one, belong to capsular type a, and toxa+ strain distributed among strains were isolated from both...
full textGenetic diversity among Pasteurella multocida strains of avian, bovine, ovine and porcine origin from England and Wales by comparative sequence analysis of the 16S rRNA gene.
Genetic diversity among 86 Pasteurella multocida isolates was investigated by comparative sequence analysis of a 1468 bp fragment of the 16S rRNA gene. The strains included 79 field isolates recovered from birds (poultry) (22), cattle (21), pigs (26) and sheep (10) within England and Wales, four Asian isolates associated with bovine haemorrhagic septicaemia, and the type strains of the three su...
full textIdentification of Escherichia coli through analysis of 16S rRNA and 16S-23S rRNA internal transcribed spacer region sequences
A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia...
full textMolecular Typing of Avian Pasteurella multocida lsolatesby PCR-RFLPof ompH Gene
Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragmentlength polymorphism (RFLP) of a species-specific PCR assay. Amplification was based on the gene ompH, encoding a major outer memberane protein. RFLP analysis of the 1.2 kb ompH-amplification usingEcoRI, HindIII and CfoI endonucleases produced 7 different patterns for the twenty fi...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of veterinary researchPublisher: shiraz university
ISSN 1728-1997
volume 9
issue 3 2008
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023